一、教师基本信息

姓名:李拓

职称:讲师、硕士研究生导师

电子邮箱:lituo@dlu.edu.cn

办公地点:大连大学问源楼A座3层

二、个人简介

男，1990年出生，博士。
1, 2008-2012, 内蒙古科技大学包头医学院药学院，学士

2, 2012-2015, 昆明理工大学生命科学与技术学院，硕士

3, 2015-2019，University of Copenhagen, Department of Drug Design and Pharmacology, 博士

4, 2019-2021，中国科学院深圳先进技术研究院，合成生物学研究所，博士后

5, 2021-2022，中国科学院深圳先进技术研究院，合成生物学研究所，助理研究员

6, 2022年10月至今 bat365官网登录入口，bat365中文官方网站，讲师
三、研究方向：天然产物化学及天然化合物的生物合成

1、古菌膜脂与植物萜类的合成生物学研究

致力于利用合成生物学技术，通过在大肠杆菌和酿酒酵母等异源宿主中表达相关基因，研究海洋来源的古菌以及植物来源的萜类化合物的生物合成机制。同时，通过代谢工程策略优化相关生物合成途径，提高具有重要经济价值萜类化合物的产量。

2、活性天然产物的图谱技术与结构研究

应用高分辨活性图谱技术探究活性天然产物（植物与微生物来源），以及分离、纯化与天然产物结构鉴定的相关技术开发与应用。

四、科研项目：
1、阿斯加德（Asgard）古菌细胞膜磷脂合成酶GGGPS底物选择性研究，国家自然科学基金青年基金（32201201），30万元，主持
2、β-榄香烯工程菌株构建及关键合酶的研究，bat365官网登录入口科研平台校内基金（202301YB04），5万元，主持

五、科研成果：

1, Bioactivity guided isolation of anticancer constituents from Hedera nepalensis K. Koch.

Tuo. Li, H. Pan, Y. Feng, H, Li, Y. Zhao. South Africa Journal of Botany. 2015, 100, 87-93.

2, Identification of a-glucosidase inhibitors in Machilus litseifolia by combined use of high-resolution a-glucosidase inhibition profiling and HPLC-PDA-HRMS-SPE-NMR.

Tuo Li, Kenneth T. Kongstad, and Dan Staerk. Journal of Natural Products. 2019, 82, 249-258.

3, Antidiabetic constituents of Dendrobium officinale as determined by high-resolution profiling of radical scavenging and a-glucosidase and a-amylase inhibition combined with HPLC-PDA-HRMS-SPE-NMR analysis.

Chu Chu, Tuo Li, Hans Albert Pedersen, Kenneth T. Kongstad, Jizhong Yan, Dan Staerk. Phytochemistry Letters. 2019, 31, 47-52.

4, Combined magnetic ligand fishing and high-resolution inhibition profiling for identification of a-glucosidase inhibitory ligands: A new screening approach based on complimentary inhibition and affinity profiles.

Sileshi G. Wubshet, Bingrui Liu, Kenneth T. Kongstad, Ulrike Böcker, Malene J. Petersen, Tuo Li, Junru Wang, Dan Staerk. Talanta 200 (2019) 279–287.

5, Structure Elucidation of Prenyl- and Geranyl-Substituted Coumarins in Gerbera piloselloides by NMR Spectroscopy, Electronic Circular Dichroism Calculations, and Single Crystal X-ray Crystallography

Tuo Li, Xue Ma, Daniil Fedotov, Louise Kjaerul, Karla Frydenvang, Sonia Coriani, Paul Robert Hansen, Kenneth T. Kongstad and Dan Staerk. Molecules 2020, 25, 1706.

6, 基于合成生物学策略的酶蛋白元件规模化挖掘,
张建志, 付立豪, 唐婷, 张嵩亚, 朱静, 李拓, 王子宁, 司同. 合成生物学, 2020,1,(3): 319-336.

7, Anti-bacterial mechanism of the P-3 against Aeromonas hydrophila NJ-35 extracted from the Potentilla chinensis Ser. (Wei Ling Cai)

Zhenzhen Li, Lili He, Liren He, Fuxing Chen, Suquan Song, Hang Zhang, Weidong Sun, Xiaowei Bao, Haibin Zhang, Tuo Li, Chenghua He, Aquaculture 541 (2021) 736746.

8, Biosynthesis of Hybrid Neutral Lipids with Archaeal and Eukaryotic Characteristics in Engineered Saccharomyces cerevisiae.

Jianzhi Zhang, Tuo Li, Zhilai Hong, Chenfei Ma, Xiaoting Fang, Fengfeng Zheng, Wenkai Teng, Chuanlun Zhang, and Tong Si. Angewandte Chemie, 14 Dec 2022, 62(4):e202214344.

9, Polypharmacology-Labeled Molecular Networking: An Analytical Technology Workflow for Accelerated Identification of Multiple Bioactive Constituents in Complex Extracts.

Yong Zhao, Oliver Gericke, Tuo Li, Louise Kjaerulff, Kenneth T. Kongstad, Allison Heskes, Birger L. Møller, Flemming S. Jørgensen, Henrietta Venter, Sonia Coriani, Susan J. Semple, and Dan Staerk, Analytical Chemistry, 2023, 95, 9, 4381–4389

10, Orthogonal Reversed-Phase C18 and Pentafluorophenyl HPLC Separation for Phytochemical Profiling of Serrulatanes in Eremophila denticulate.

Yong Zhao, Tuo Li, Louise Kjaerulff, Henrietta Venter, Sonia Coriani, Birger Lindberg Møller, Susan Semple, and Dan Staerk. Journal of Natural Products. 2023, 86, 12, 2638–2650

11, Two-phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes.

Tuo Li, Ximeng Liu, Haoyu Xiang, Hehua Zhu, Xuan Lu, and Baomin Feng. Molecules 2023 (accepted)